Genetic Transformation and Hairy Root Induction Enhance the Antioxidant Potential of Lactuca serriola L.

Cannabis sativa L. genetically transformed root based culture via Agrobacterium rhizogenes

It is an increased interest in the therapeutic potential of Cannabis sativa L. (marijuana) for treatment of multiple sclerosis and HIV neuropathy. Because of limitation in cultivation of this plant, an efficient hairy root induction system for Cannabis sativa L. was developed in the present study. Agrobacterium rhizogenes mediated transformation performed by two different co-cultivation mediums...

Effect of elicitation on antioxidant activity and production of tropane alkaloids in Hyoscyamus reticulatus hairy root cultures

Background and objectives: Hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. In the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in Hyoscyamus reticulatus. M...

This study evaluated the induction of hairy roots in Perovskia abrotanoides Karel, a medicinally important member of Lamiaceae family, using different strains of Agrobacterium rhizogenes and various carbon supplies, for the first time. This research established an efficient Agrobacterium mediated transformation system for this plant species through bacterial strains including ATCC15834, TR105 a...

Induction of DrsB1-CBDAvr4 Recombinant Protein in Hairy and Adventitious Roots of T1 Transgenic Plants

Hairy and adventitious roots are efficient systems for expressing recombinant proteins. In the present study, the amount of DrsB1-CBDAvr4 recombinant protein in hairy and adventitious root systems was compared. To this end, the effect of different factors on the optimization of culture conditions to obtain adventitious and hairy roots was evaluated in three separate experiments by assessment of...

Secondary Metabolite Contents and Antioxidant Enzyme Activities of Cichorium intybus Hairy Roots in Response to Zinc

Hairy root systems are formed by transforming plant tissues with the “natural genetic engineer” Agrobacterium rhizogenes. In most plants such as Cichorium intybus L., hairy root cultures have proven to be an efficient system for secondary metabolites production. The effect of Zinc (ZnSO4), a heavy metal, was investigated at different concentrations (0, 1, 5 and 10 mM) on some secondary metaboli...